Monday, June 23, 2014

P. larvae Project Pilot

Checked plates containing P. larvae. Will be ready to attempt to harvest spores tomorrow if I have enough time in between my bee rearing duties and I am able to procure adequate equipment.

          
P. larvae on MYPGP                               P. larvae on Chocolate Agar

Below are images of P. larvae that was plated onto MYPGP agar. Cultures were diluted in sterile ddH2O from broth culture of P. larvae. Note: 100 uL broth culture + 900 uL ddH20 = 1:10 dilution, plate 100 uL of that onto agar + 1:100 dilution.

       
P. larvae diluted 1:100 on MYPGP          P. larvae diluted 1:1000 on MYPGP 

//EWW

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