Wednesday, December 3, 2014
Tripartite Conjugation in P. larvae
The goal of this project is to develop a mutagenesis procedure in P. larvae 9545 that will randomly insert transposons into its genome. This particular method has been shown to be effective in other Bacillus spp..
December 2nd, 2014
Brothed:
P. larvae 9545 into 10 mL of BHI + thiamine (target genome)
EC pMarA into 10 mL of BHI Amp100 and Amp100 + Kan50 (transposon)
EC pRK2013 into 10 mL of BHI Kan 50 (helper plasmid)
//EWW
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