Goal: Perform chlorine dioxide pilot study using the 50 mL conical tubes as before except I will be using Bacillus subtilis and Bacillus thuringiensis spores that I have recently extracted using the heating/washing procedure.
Sterile glass cover slips were aseptically transferred to petri plates in the fume hood and a volume of 25 uL of spores from either Bacillus spp. were added to the center of the glass side. The slides were left in the fume hood for 30 minutes or until the spores had evaporated onto the slides.
//EWW
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