Adhesion Toxicity Assay 24 hours

Continuation of Adhesion Toxicity Assay started on 5-9-14

List of Treatment Groups:

Treatment Group	Description	Image	Website
A	New-Skin™ Liquid Bandage	Link	Website
B	Krazy™ Glue	Link	Website
C	Loctite™ Super Glue	Link	Website
D	E600 Industrial Strength Adhesive	Link	Website
E	Aleene's™ Super Gel Adhesive	Link	Website
F	Gorilla™ Super Glue	Link	Website
G	Beacon™ 527 Multi-Use Glue	Link	Website
H	Weldbond™	Link	Website
I	Scotch™ Maximum Strength Adhesive	Link	Website
J	Elmer's™ China + Glass Cement	Link	Website
K	Elmer's™ Glue-All	Link	Website
L	Control (No adhesive)	N/A	N/A

24 hours after application of the adhesives to the dubia roaches:

Roaches removed from 37 C and dead/alive status was determined along with additional noticeable status ailments.

Table of Results at 24 hours

Treatment	Dead (out of 8)	Additional Comments
A	6	The two remaining alive roaches displayed lethargy and are near death
B	1	All alive roaches look to be in healthy and lively condition
C	0	All roaches look to be of good health
D	1	Remaining seven alive roaches display lethargy
E	1	Two of the seven alive roaches had visible precipitate covering their body (see Image 1 below)
F	1	Light visible precipitate on most of the seven alive roaches, not as noticeable as with Treatment E
G	0	Some of the alive roaches displayed lethargy
H	0	All roaches displayed lively health, one had molted while in containment (see Image 2 below)
I	1	All seven alive roaches displayed lethargy
J	0	All roaches displayed lively health
K	0	All roaches displayed lively health
L	0	All roaches displayed lively health

Image 1. Visible adhesive precipitate on two of the eight roaches in the Treatment E group. Both of these roaches were in the same treatment group. The bottom roach (along with one other) had a coating of white covering its entire body. Microscopic visualization of the surface of those two roaches looked like tiny white crystals attached to their body.

Image 2. Recently molted roach in Treatment H. Underneath the white colored roach in the image below is its molted exoskeleton. There is only one roach in this mini-cup.

All roaches, along with those that had died were returned to their sealed containers as before and incubated at 37 C once again. Survival status will be monitored at 48 hours.

Discussion:

So far, there hasn't been a lot of death associated with different types of adhesives expect in Treatment A, which was the New-Skin™ Liquid Bandage. This adhesive did contain 8-Hydroxyquinoline antibiotic as an active ingredient and is obviously considered non-toxic to humans. Unfortunately, this was the only liquid-bandage type adhesive we have tried in this particular study simply because it was the only brand we were able to locate. Ideally, we would like to test other liquid-bandage type adhesives without antibiotics in the future. It is a mystery as of right now why two of the eight roaches in Treatment E were coated with a precipitate. This could be due to unequal amounts of adhesive that was added to those two in particular or related to the fumes that were thrown off by the adhesive due to a larger amount being present. Perhaps the adhesive wasn't completely dried and was transferred to the mini-cups where the roaches' body came in contact with it. Additionally, it was unfortunate that one of the roaches in Treatment H molted during this study. That alters the consistency of this study in that Treatment group. That was one concern of ours while using the juvenile dubia roaches, but unfortunately we were limited to what was available as we needed a large amount of them.

There are a number of treatment groups (C, J, and K) that have so far resulted in no deaths or lethargy in the roaches. Treatment groups will continue to be monitored for 168 hours (7 days). The active ingredients of each adhesive will be more closely examined in the future. There is likely a relation between active/inactive ingredients and toxicity to the dubia roaches.

//EWW