Shadowed an RNA Extraction method (procedure not shown) with DeeptiT. of the T.Bergholz lab. Attempted to extract RNA from two S. maltophilia strains (B2423 and B84). This method doesn't make use of a kit or kit reagents, so if successful this method could possibly be included in a future methods paper for S. maltophilia.
Bacterial strains were grown to stationary phase in 5 mL of LB broth at 37C 225 rpm before beginning the procedure.
Nucleotide samples were stored at -80C overnight before DNAse treatment tomorrow.
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