DeeptiT. of the T.Bergholz lab checked the purity of the extracted RNA from the two S. maltophilia isolates. The results below are of the extracted nucleic acids without DNAse being added yet.
|
Isolate
|
260
|
280
|
260/280
|
260/230
|
ng/ml
|
|
B24
|
58.0
|
29
|
2.00
|
1.97
|
2320.7
|
|
B84
|
47
|
23.5
|
2.00
|
1.94
|
1824.0
|
Purity and concentration look excellent. However, this is before DNAse was added, so we could still be detecting DNA in the sample. DNAse will be added and samples will be read again in the near future.
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