Thursday, June 19, 2014

P. larvae Project Pilot

Growth observed on MYPGP and Chocolate Agar. More growth on Chocolate agar observed.

Brothed an isolated colony of P. larvae from chocolate agar into 10 ml of BHI broth with 1ug/ml of Thiamine. Incubated at 37C 5% CO2 concentration. Will make freezer stocks tomorrow. Will also perform Gram stain to confirm Gram positive is present.

Continued to incubate P. larvae plates until sporulation occurs in another ~4 days.

//EWW

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