RNA Extraction

DeeptiT. of the T.Bergholz lab performed the DNase treatment to the extracted RNA and then quantified each on the nanodrop.

Isolate	260	280	260/280	260/230	ng/ml
B24	47.2	24	1.99	2.22	1887.1
B84	58	29.4	1.97	2.19	2320.2

We decided not to examine the RNA samples on the Bioanalyzer to check if the RNA was indeed good quality. It was determined that this RNA Extraction procedure does indeed work well for S. maltohpilia and will be included in a possible future methods publication. We currently have no use for RNA from S. maltophilia, but it is still useful to know of a method that works for its extraction that doesn't cost a lot of money (as opposed to using a kit) should we ever be in need of a large quantity of RNA.

//EWW