Repeat of the previous ClO2 gas exposure experiment using the late instar wax worms. I exposed 10 wax worms in each container to either no ClO2 gas, 100 mg (total reagent weight), or 200 mg. The reagents were mixed in the modified PCR tubes (six small holes added to them using a heated inoculating needle). The 925 mL volume containers were used again.
Three different conditions:
100 mg
200 mg
No gas
After the PCR tubes containing the ClO2 reagents were added to the containers they were sealed with their covers and wrapped using Parafilm to ensure the gas could not escape. Containers were incubated at room temperature, protected from light for 24 hours.
I will repeat the procedure I did last time, where I remove the PCR tubes after 24 hours, vent off any remaining gas using the laminar flow hood, and allow the wax worms to recover for an additional 24 hours before determining survival. I will also count the number of wax worms that have formed cocoons (if any) after the initial 24 hours.
//EWW
No comments:
Post a Comment