ClO2 generation in chambers

Goal: Determine the rate at which ClO2 gas is being generated inside the modified anaerobic chambers. This information will be useful information for my ClO2 exposure study as an effective was of killing P. larva spores on apiary-related surfaces. Based on preliminary results, there appears to be a parabolic trend associated with gas generation inside the chambers, with Cl ppm peaking around 3 hours.

For this study, the modified anaerobic chambers were set up like they would be for the spore-exposure study (50 mL H2O, stir bar, etc.). A total weight of ClO2 reagents of 100 mg (50 mg from each Part A and Part B) was used for these experiments. The reagents were mixed inside a PCR tube and after mixing the tube was placed, open, inside the chambers. The ppm of Cl was checked every 30 minutes and were recorded.

Time	ppm Cl
0.5 hr	65
1.0 hr	75
1.5 hr	90
2.0 hr	100
2.5 hr	105
3.0 hr	105

I will continue the experiment with time points after 3.0 hrs up to 6.0 hours. 

//EWW