From 7-31-14.
PCR performed on several colonies recovered from homogenized honey bee larva. AFB-F/R primers used. Loaded amplicons (2uL EZVision + 7uL DNA) into a 1% gel. Ran electrophoresis at 120V for 0.5 hour.
There was no amplification of any of the colonies recovered from the homogenized larva tested positive using the AFB primers (Gel not shown). Positive control checked out. It could be that whatever the bacteria colony was outcompeted the slow growing P. larvae. I am not sure how to proceed with this study at this moment. It will likely be put on hold for the time being to focus on more... lucrative experiments.
//EWW
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