From 10-28-14.
I was unable to count any colonies from the two treatment groups on the MYPGP plate from two days ago. There were no colonies observed on the 10^0 dilution for both the control and experimental (ClO2) treatment groups. This likely indicates that the samples were diluted out beyond observation. Only 50 uL of broth culture was affixed to a glass cover slip, and then that was re-suspended in 10 mL of ddH2O, that is a 1:200 dilution right away. Also, the broth culture consisted of mostly vegetative cells, which likely died during the drying process. It is not terribly odd that no colonies were observed in my control treatment when considering that fact.
In the future, if I am interested in repeating this experiment for TOTAL cells, what I could do, and should have done this time, was centrifuge down the broth culture to a pellet and then re-suspend the pellet in a smaller volume as to concentrate my sample before affixing to the cover slip. That way I'd have a higher initial concentration and the effects of diluting and die off will be less devastating.
In the mean time, the experiment was repeated as before, except this time P. larvae 9545 spores were used in place of the broth culture. Specifically 50 uL of Spore Stock E was added to each cover slip. Spores were dried onto the cover slip as before and exposed to ClO2 at the same concentration (86 mg of each component). Incubated for six hours at room temperature slightly shaking on a shaking incubator.
//EWW
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