Strains for Sequencing

From 10-17-14.

Digested gDNA with EcoR1 using EcoR-HF with Cutsmart buffer from NEB. Digests performed at 37C for 25 minutes. Digested and undigested DNA was mixed with EZ loading dye and loaded into a 1% agarose gel. Gels were ran at 140 V for 1 hour.

Gel image results:

Digest Gel 1

Lane 1	Lane 2	Lane 3	Lane 4	Lane 5	Lane 6	Lane 7	Lane 8
HiLo	1 cut	1	4 cut	4	5 cut	5	9 cut

Digest Gel 2

Lane 1	Lane 2	Lane 3	Lane 4	Lane 5	Lane 6	Lane 7	Lane 8
HiLo	6 cut	6	7 cut	7	8 cut	8	9

Key

1	P. larvae 9545
4	4D1
5	6A5T
6	13A1
7	13A4
8	4AA1
9	13A82

It appears that all gDNA was successfully digested into unique bands and that the gDNA alone was one band. This indicates that the gDNA is what we think it is - gDNA. In the very near future, about four of these samples will sent for sequencing.

//EWW