Made freezer stocks of the majority of the Bacillus Stock Center isolates. Overnight cultures were combined with glycerol in a freezer tube and stored at -80C in the orange spore stock box.
800 uL of overnight broth culture + 200 uL of 80% glycerol
Freezer stocks made:
4Q1 - Bacillus thuringiensis subsp. israelensis ONR60A
30A1 - Paenibacillus dendritiformus subsp. dendron T168
30A2 - Paenibacillus dendritiformus subsp. charalis C168
33A1 - Paenibacillus alvei III3DT-1A
33A2 - Paenibacillus alvei III2E
40A1- Brevibacillus laterosporus ATCC 9141
40A2 - Brevibacillus latersporus ATCC 6457
40A6 - Brevibacillus latersporus NRRL B-4189
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| Freezer Stock Manifest |
I was not able to make a freezer stock of 26A3, Brevibacillus formosus SS 8604, as it did not grow on the NYSM agar. Currently it is still located on two discs at 4C. I may in the near future attempt to culture this bacterium again.
//EWW
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