Prepared media in order to make freezer stocks of the remaining spores from the Bacillus Stock Center shipment. The spores have since been stored at 4C.
Nutrient Agar and Nutrient Broth was prepared.
NYSM Agar was prepared as well (Link).
ATCC medium: 2302 NYSM medium
Nutrient agar...............23.0 g
Yeast extract................0.5 g
MnCl2 ........................9.9 mg
CaCl2 . 2H2O ................10.3 mg
MgCl2 . 6H2O ...............203.3 mg
Distilled water..............1.0
Autoclave at 121C for 15 minutes.
Media was autoclaved for 20 minutes.
Strains:
4Q1 - Bacillus thuringiensis subsp. israelensis ONR60A
26A3 - Brevibacillus formosus SS 8604
30A1 - Paenibacillus dendritiformus subsp. dendron T168
30A2 - Paenibacillus dendritiformus subsp. charalis C168
33A1 - Paenibacillus alvei III3DT-1A
33A2 - Paenibacillus alvei III2E
40A1- Brevibacillus laterosporus ATCC 9141
40A2 - Brevibacillus latersporus ATCC 6457
40A6 - Brevibacillus latersporus NRRL B-4189
Media plated on for each strain:
4Q1 - LB agar
26A3 - NYSM agar
30A1 - LB agar
30A2 - LB agar
33A1 - NYSM agar
33A2 - NYSM agar
40A1- Nutrient agar
40A2 - Nutrient agar
40A6 - Nutrient agar
Discs were aseptically transferred to the center of their appropriate agar media and were saturated with a small amount of appropriate broth media. Plates were incubated at 37C not inverted for 24 hours.
//EWW
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