Chlorine Dioxide Pilot Study

From 10-8-14.

Repeated chlorine dioxide water pilot from last week. This time a small fan (Ultra-Mini Fan from Go Travel) was added to each anaerobic container and the two chemical components were combined in a small sachet instead of a PCR tube as performed before. Additionally, a humidity and temperature detector was added to the last container in each treatment group. Temperature and humidity was measured at each of the six time points for each treatment group.

Ultra-Mini Fans from Go Travel that were used in this experiment

Each anaerobic chamber was set up similar to the picture below. The fans were afixed the bottom of the chamber using double sided tape so they would not fall over and remained at a fixed location throughout the experiment. Only the six hour treatment had the temperature/humidity gauge in it.

Anaerobic plate set up for this experiment

Below are better images of the nozzle that was put into the covers of the anaerobic chambers. Each chamber had a single nozzle placed in them by drilling a small hole and gluing the nozzle into place.

Below are the raw data results from the Cl2 gas column gauge. Also included is the temperature and humidity at each time point. Because of how the experiment was set up, 200 mL of gas was removed only once from each container.

Raw Data	10/18/14
	Hour 1	Hour 2	Hour 3	Hour 4	Hour 5	Hour 6
No Water	75	60	50	50	45	40
Temp	22.8 C	22.7 C	22.5 C	22.2 C	22.1 C	22.2 C
Humidity	30%	30%	30%	31%	31%	31%
Water	100	95	95	80	70	50
Temp	22.6 C	22.4 C	22.1 C	22.1 C	22.2 C	22.0 C
Humidity	98%	98%	98%	94%	92%	90%
Correction Factor of 0.25
	Hour 1	Hour 2	Hour 3	Hour 4	Hour 5	Hour 6
No Water	18.75	15	12.5	12.5	11.25	10
Water	25	23.75	23.75	20	17.5	12.5

//EWW