Stocks from Bacillus Genetic Stock Center

From 10-6-14.

Made freezer stocks of the five E. coli strains that were incubated overnight in LB Abx at 37C 225 rpm.

800 uL of broth culture + 200 uL of 80% Glycerol in a freezer tube

Made freezer stocks all in triplicate. Place in orange tube box and wrote "Fisher Spore Box" on it. The box was stored in the -80C freezer on the top shelf.

Plated several of the spore forming bacteria that also arrived from the Bacillus Genetic Stock Center.

Spores were soaked into small discs present in wrapped aluminum foil. These small discs were transferred to LB agar using sterile forceps (flamed to sterilize). Three drops of LB media were carefully added to the disc that was present on the LB agar. Plates were incubated at 30C in the walk in incubator, not inverted.

Strains that were plated onto LB

4D1 Bacillus thuringiensis 

4AA1 Bacillus thuringiensis

6A5T Bacillus cereus

13A1 Lysinibacillus sphaericus

13A4 Lysinibacillus sphaericus

13A82 Lysinibacillus sphaericus

//EWW