9-16-14
The three P. larvae spread plates have been incubating at 37C 5% CO2 for seven days. Spores from these plates were collected using the method afore described. There were fewer visible colonies that previously seen before after seven days of incubation of P. larvae. Regardless, spore preps were performed and the final spore pellet was resuspended in 500 uL of sterile ddH2O.
Performed the drop plate method on MYPGP to quantify CFU/mL in the three P. larvae spore stocks. Also performed this method on LB only using the B. thuringiensis spore stock. P. larvae plates were incubated at 37C 5%CO2 and B. thuringiensis plates were incubated at 30C.
Today
B. thuringiensis plates were checked after about 18 hours and had countable colony formations.
B. thur Spore Stock Dilution
|
||
10^-8
|
10^-9
|
10^-10
|
15
|
1
|
0
|
20
|
2
|
1
|
21
|
2
|
0
|
18
|
3
|
0
|
14
|
2
|
0
|
17.6
|
2
|
0.2
|
The bold underline numbers are the averages from each dilution that was plated out.
Determining CFU/mL from colony count formula/equation:
[# colonies counted / volume pipetted onto plate in uL] * [ 1 / serial dilution made (ie 10^-2)] * [1000 uL / 1 mL] = CFU/mL
My stock spores are resuspended in 500 uL of ddH2O so,
Concentration of the B. thuringiensis is 3.52x10^11 CFU/500 uL
//EWW
No comments:
Post a Comment