Having some difficulty quantifying the concentration of my B. thuringiensis spore stock. I was unable to quantify any accurate colony forming units from the prepared dilutions, they are usually one giant colony at 24 hours of incubation at 30C and contained a lot of "contamination" that wasn't from the 10uL drop. A picture below shows the trails of what appears to be B. thruingienisis throughout the plate. The trails appear to follow the path the pipet made to drop the 10uL onto the plate. I am not sure if this is because the spores were falling out of the pipet as it was brought to the drop location or what other possible reason there could be. It is perplexing; this has not happened with any other bacterial strain while I have performed this method. Perhaps I will attempt to do a hemocytometer count of the spores in order to quantify their concentration.
Inventory was performed of the supplies that has been received from the USDA so far for the chlorine dioxide study. A picture of all the boxes that have been received is seen on 9-8-14.
Petri Plates
100 x 15 mm (normal size) - 2 boxes (500 plates/box)
150 x 15 mm (larger size) - 5 boxes (100 plates/box)
Chemicals
Yeast Extract - 1000 g
Mueller Hinton Broth - 500 g
Potassium Phosphate Dibasic - 100 g
D-(+)-Glucose - 100 g
Sodium Pyruvate - 100 g
Equipment
AnaeroPacks (Anaerobic chambers) - 8 containers
VWR Cell Spreaders - 50 packs (500 total spreaders)
Plates were stored in the Fisher lab on the high shelves along with the anaerobic chambers. Most of the chemical were assimilated into the rest of Fisher lab chemicals in the large shelving unit.
//EWW
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