I've experimented with a new type of Potato-Honey agar to potentially be used as a media for the P. larvae LD50 assay. The recipe was found on an online blog from some guy who raises wax worms in his basement.
Potato-Honey (PH) Agar (1 Liter)
20 g Agar
10 g Instant Potato Flake
20 mL Honey
(Sterilize in Microwave)
A volume of 125 mL of PH agar was made in a 250 mL flask. The solution was microwaved until the potato flakes dissolved and became homogenous (or close to it). The solution was periodically removed from the microwave and stirred by hand. After the PH agar was microwaved a small portion was transferred to the wells of a 96 well plate using a sterile eye dropper. This method seemed to work well and the agar solidified nicely in the wells. My only concern is how sterile the PH agar is and if something will begin to grow in the agar. Remaining PH agar was poured into petri plates, it'd be interesting to see if WW eggs would hatch on this surface...
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| Side view of 96 well plate with PH agar in the wells |
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| Bottom of 96 well plate with PH agar in it |
PH agar is very dense and heavy compared to other agar types (like Nutrient Agar or LB Agar). It isn't as soft and has a rather solid consistency thanks to the presence of the honey.
//EWW
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