I was able to count CFU from the MYPGP plates that had the spores that were exposed to ClO2 gas for three hours on them. Spore stock #10 (1.1x10^7 CFU/mL) was initially inoculated onto the glass cover slips. Since a volume of 100 uL was added to the cover slips that means there is a concentration of 1.1x10^6 CFU on them before they were exposed to ClO2.
| ClO2 Reagent | CFU/mL |
| 0 mg | 9.33E+05 |
| 50 mg | 6.67E+05 |
| 100 mg | 5.67E+05 |
| 200 mg | 3.67E+05 |
There is a decreasing trend seen in spore survival as you increase the concentration of ClO2 reagents. This is a similar trend to what has been seen before after exposing for 6 hours, however the log killing was higher (due to the increase amount of exposure time). The standard deviations aren't very good, likely due to subtle decreases in spores between concentrations. For example, when 200 mg were used to treat spores on glass for 6 hours there was over a 3 log killing rate. It would appear that the length of exposure time has an effect on killing of spores, as well as generation of gas.
//EWW
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