ClO2 Study - P. larvae Spores on Glass

From 4-19-15.

Repeated ClO2 exposure to P. larvae spores. A lower concentration of ClO2 reagents was used in this experiment. Spore stock #16 was used for the adhesion onto glass cover slips. The experiment was performed exactly as before (same setup) except a total weight of 12.5 mg of ClO2 reagent was used (~6.25 mg each). The reagents were mixed inside PCR tube as before and, surprisingly, appeared to mix well with each other.

I also performed another humidity & temperature check inside the modified anaerobic chambers after 6 hours.

Temperature, Humidity

28.7C, 96% (today)
28.8C, 95% (today)

28.8C, 96%(from 4-19-15)

The temperature and humidity inside the modified anaerobic chambers appear to be consistent between days, different chambers, and the different magnetic stir plates. 

Below is a graph of the cumulative results so far with the spores on the glass surface. I will know the CFU/mL of spores after they've been exposed to 12.5 mg of spores after this experiment today.

After the six hours have been reached using each different weight of ClO2 reagent I have taken a 200 mL sample using the the Cl columns to detect the ppm of Cl gas inside the chamber. Below is graph and data table containing the determined ppm of Cl for each dry weight of reagents used.

Weight	Cl ppm
200 mg	75
100 mg	55
50 mg	45
25 mg	25
12.5 mg	15
0 mg	0

//EWW