It has been ten days that the P. larvae 9545 has been incubating on the blood agar slants at 37C. Today I extracted the spores from the slants as previously performed (washing, heating, and centrifuging). Five slants were consolidated together into one spore stock.
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| One of the dilution plates from the spore stocks |
I was also able to quantify the CFU/mL of the P. larvae spore stocks that were from the blood agar plates. Below is there determined concentration:
| Stock # | Source | CFU/mL | CFU/mL |
| 17 | Plate | 1600000 | 1.6x10^6 |
| 18 | Plate | 3600000 | 3.6x10^6 |
Other spore stocks here.
How to calculate CFU/mL
(CFU * Dilution Factor * Volume Factor)
or
(# CFU) * ( 1 / 10^ dilution factor) * ( 1 / volume plated in mL) = CFU / mL
ex:
3 * ( 1 / 10^ (-4)) * ( 1 / 0.01mL) = 3x10^6 CFU /mL
//EWW
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