Sporulation on Blood Agar

From 4-3-15.

I was able to calculate the CFU/mL of the P. larvae spores recovered from the Columbian sheep's blood agar plates and slants. The colonies were grown on MYPGP agar at 37C for three days before they were counted. The spore concentrations aren't as a high as I would have liked (aiming for a 10^8 CFU/mL), but through use of the blood agar the concatenation of spores is about 1 to 2 logs higher. It appears that the bacteria growing on either the slant or the agar didn't seem to make much difference.

Stock #	Source	CFU/mL	CFU/mL
10	Plate	11000000	1.1x10^7
11	Plate	1800000	1.8x10^6
12	Plate	600000	6.0x10^5
13	Slant	800000	8.0x10^5
14	Slant	1600000	1.6x10^6
15	Slant	2100000	2.1x10^6
16	Slant	2800000	2.8x10^6

I plan to use these spore stocks for the upcoming chlorine dioxide experiment.

//EWW