Since I am apparently having so many issues with amplifying the 16S region of the selected P. larvae honey isolates I have decided to go back to the drawing board and re-streak out the cultures from freezer stock and try again. I did not use the same exact isolates as last time in all cases, but I did have representatives from the five different honeys that were screened and found to contain P. larvae.
Isolates stuck out on MYPGP & LB agar
dd1-5a
dd5-4a
dd8-55a
dd11-2a
dd12-22a
The dd11-2a and dd12-22a are the same isolates previously examined as they were the only representative for their honey source that was seen to have an amplification using the AFB primers.
//EWW
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