Detection of P. larvae in Local Honey

From 1-5-15.

There was visible growth in all the remaining honey isolates except for isolate number 12, which still had that mysterious globule formations (see previous post). Freezer stocks were temporarily stored at -20C for the time being.

The only isolate from the original honey bee samples that I was not able to make freezer stocks from was isolate number 12 (dd8-85a). However, isolate number 11 was also dd8-85a and was most likely a clone of the isolate 12.

Made isolation streak plates of representative P. larvae isolates from five of local honey samples. There were only five different honey sources that P. larvae isolates were identified from. Isolates were struck out onto MYPGP agar and incubated at 37C. Those isolates were:

dd1-8a

dd5-Q

dd8-63a

dd11-2a

dd12-22a

I plan to extract genomic DNA of these isolates and then sequence their 16S DNA to confirm their identity.

//EWW