From 12-24-14.
Goal: Make freezer stocks of the new P. larvae isolates from local honey.
12-31-14 Wednesday
Inoculated each of the 30 PCR-confirmed P. larvae isolates into 10 mL of BHI broth and incubated at 37C 225 rpm.
1-1-15 Thursday
Most of the cultures had not grown yet. The media was still a clear color. This may be due to the fact that the plates that the isolates were transferred from had been in the 4C fridge for over a week. The cultures may just need more time to incubate, as they are relatively slow growing bacteria. The cultures continue to incubate at 37C 225 rpm.
1-2-15 Friday
The majority of the cultures had visible growth. Freezer stocks were made of these cultures
Freezer stocks: 200 uL 80% glycerine + 800 uL broth culture
The other cultures that had no visible growth were continued to incubate at 37C 225rpm. If no visible growth is observed tomorrow, I will re-inoculate the culture from the plates stored at 4C.
//EWW
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