Sunday, January 18, 2015

Chlorine Dioxide Pilot Study

From 11-9-14.

Gear up to perform another pilot study to determine if chlorine dioxide gas has any effect at all on killing P. larvae spores.

First I need to quantify the concentration (cfu/ml) of P. larvae spores that I have in my previously created spore stocks. Spore stocks C, D, E and spore stock created on 11-4-14 (called F, G, H, I, J) were diluted 1:10 in a 96 well plate (10 uL  --> 90 uL) down to 10^-4. A volume of 10 uL from each dilution was plated onto MYPGP agar and incubated at 37C.

//EWW

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