Detection of P. larvae in Local Honey

From 1-19-15.

Double checked the primers 8F and 1492R and they were correct and so was the PCR parameters from Turner et al. 1999. These parameters were said to have resulted in amplification of the 16S using the 8F and 1492R primers that are also described in the Turner paper. So, it would appear that the primers themselves nor the thermocycler parameters were to blame for the blank gel.

I re-ran the amplicons, loading twice the amount of DNA and also adding EZ vision loading dye, however it still resulted in a blank gel, meaning that the DNA did not amplify. This is possibly because there wasn't enough or any DNA template for the reaction to occur.


//EWW