Sunday, January 18, 2015

Detection of P. larvae in Local Honey

From 1-12-15.

Performed PCR on the six isolates: dd1-8a, dd5-Q, dd8-63a, dd11-2a,dd12-22a, and the roach trachea isolated using the primers 8F and 1492R and the thermocycler parameters previously described. 

Created a 1% agarose gel (0.5 g + 50 mL TAE buffer) and stored it at 4C until use tomorrow.

//EWW

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