Detection of P. larvae in Local Honey

From 1-11-15.

Created a thermocycler program for the new primers 8F and 1492R based on parameters described by Turner et. al 1999.

The program was named "8F" after the forward primer. The parameters are as follows:

1. 95C - 3 minutes

2. 95C - 15 seconds

3. 49C - 15 seconds

4. 42C - 90 seconds

5. Repeat 2-4 35x

6. 72C - 5 minutes

7. 20C - forever

//EWW