From 1-22-15.
Ran 1% gel with AFB amplicons from the gDNA and the gel was blank again. So, enough messing around with this gDNA as it is apparently a poor template and I likely made a mistake during the genomic DNA extraction process. I am uncertain what step a mistake was made at. It was likely during the pre-treatment of Gram positive bacteria that was performed. I will double check the method again and repeat it with more stringency in the near future.
I have already struck out the cultures from freezer stocks and am waiting for them to grow at 37C.
//EWW
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