The 1:1:1 mixture tubes and inoculated filter discs had been incubating at three different temperatures overnight. 100 uL spread plates were made of each broth mixture onto MYPGP Poly60 Kan50 plates. Plates were inverted and incubated at 37C. It may take a few days before visible colonies are observed.
The inoculated discs were aseptically removed from the agar plates using sterile forceps and transferred to 5 mL of ddH2O. Tubes were vortexed briefly to dislodge bacteria from the disc and 100 uL spread plates were made of the suspension on MYPGP Poly60 Kan50. Plates were also inverted and incubated at 37C.
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| pMarA plasmid from Le Brenton 2006. |
Interestingly, the P. larvae 9545 that was inoculated into BHI Poly60 two days ago and that has since been incubating at 37C 225rpm now has visible growth. Yesterday there was no growth that could be seen with the naked eye, however P. larvae grown in BHI only did have visible growth. It appears that the antibiotic slows the growth of P. larvae.
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| Growth of P. larva 9545 in BHI Poly60 |
//EWW
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