Detection of P. larvae in Local Honey

From 1-2-15.

All of the remaining cultures that were left at 37C 225rpm to grow still did not have visible growth.
List of isolates that did not grow:

EW code:

01

03

04

05

09

11

12

13

So, the cultures were re-inoculated. Unfortunately, not many (almost none) visible colonies remained on the old plates stored at 4C. So, 5 mL of BHI broth was flooded onto the agar plates and the top of the agar was agitated using a sterile cell spreader (image below).

The inoculated broth cultures were transferred to a 15 mL tube and incubated at 37C 225 rpm. It will likely take a couple days before visible growth is observed. I will make freezer stocks of these cultures tomorrow.

//EWW