ClO2 Study - P. larvae Spores on Glass

From 4-14-15.

Repeated ClO2 gas exposure to P. larvae spores on glass cover slips as before using different weights of ClO2 reagents. The previous time this experiment was ran I used a lot of ClO2 reagents for the reactions (200 mg and 2000 mg) which resulted in a lot of spore killing as evident by the lack of colony formation on the MYPGP agar after. The 2000 mg weight actually resulted in total killing of the spores and the 200 mg almost had the same result. So, this time I will be using less ClO2 reagent.

The total dry weights of ClO2 reagents used in this experiment were 100 mg, 50 mg, 25 mg, and 0 mg. P. larvae spore stock #15 was used in this experiment.

The spores on the glass cover slips were exposed to the ClO2 gas inside the modified anaerobic chambers inside the fume hood for six hours once again.

The spores were re-suspended after and plated on MYPGP agar as before. Plates were incubated at 37C for three days.

//EWW