Sporulation on Blood Agar

From 4-18-15.

It has been seven days that P. larvae ATCC 9545 has been incubating on blood agar at 37C with 5% CO2. The spores were extracted from the plates as previously performed (washing and heating at 60C). The newly created spore stock #17 and #18 were diluted and plated on MYPGP agar for colony counts. The plates were incubated at 37C and will remain there for three days until colonies form.

//EWW