The plates that had been struck with the P. larvae isolates from local honey samples had been incubating for three days at 37C and now have good colony formation. Interestingly, there was no growth on the LB agar plates and each isolate had growth on the MYPGP agar. As the isolates are P. larvae, they should grow well on MYPGP agar.
Isolates stuck out on MYPGP & LB agar
dd1-5a
dd5-4a
dd8-55a
dd11-2a
dd12-22a
Crude genomic lysate was made of each isolate and will be used as template DNA for a PCR reaction using the AFB primers to confirm that they are (most likely) indeed P. larvae.
An isolated colony from each isolate was inoculated into 10 mL of BHI broth and incubated at 37C shaking 225 rpm. I plan to inoculate a broth culture of these isolates onto Columbian blood agar slants and extract their spores. These spores will be used in my chlorine dioxide study. I also plan on amplifying the 16S gene's of these isolates and send them in for sequencing.
//EWW
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