Wax Worm/P. larvae LD50

From 4-18-15.

Unfortunately, some of the wax worms were able to escape the 96 well with the transparent cover, but not nearly as many as before when using the metal cover. It is still somewhat difficult to determine if the wax worm  in each well is alive or dead as sometimes they are indeed alive, but just not moving at the moment (and there are 96 of them to count!). So, I've taken to marking the well with a colored marker if they are dead and I will use a different marker each day. An "X" will denote dead and an "O" will represent that the well was empty.

I set up two additional LD50 experiments in the same way that the last one was designed. Early instar wax worms were placed into the wells of a 96 well plate containing a piece of oat with endospores. The wells were sealed with the transparent film once again and a small hole was pierced in it for each well. The plates are incubating at 37C and I will monitor survival each day.

//EWW