ClO2 Study - P. larvae Spores on Glass

From 4-11-15.

Visible P. larvae colonies have begun to form on the MYPGP agar

How to calculate CFU/mL

(CFU * Dilution Factor * Volume Factor)

or

(# CFU) * ( 1 / 10^ dilution factor) * ( 1 / volume plated in mL) = CFU / mL

ex:

3 * ( 1 / 10^ (-4)) *  ( 1 / 0.01mL) = 3x10^6 CFU /mL


Unfortunately, there were no colony growth on the 2000 mg ClO2 weight concentration, even at the least dilute plate (10^-1). This isn't overly surprising as that concentration was relatively very high. So high in fact that it turned the plastic of the anaerobic chamber and the water inside a yellow tint. Fortunately, the yellow tint has disappeared from the container after a couple of days.
There were countable colonies in the No ClO2 and 200 mg treatment groups. Below is a graph illustrating the differences between CFU in those treatment groups:

Raw Data

Colony Counts:

	Dilution Factor
ClO2 Conc	0	-1	-2	-3	-4
None	TNTC	TNTC	9	3	0
	TNTC	TNTC	9	1	0
	TNTC	TNTC	11	9	0
	TNTC	TNTC	13	3	0
	TNTC	TNTC	9	2	0
	TNTC	TNTC	13	2	0
	TNTC	TNTC	16	3	0
	TNTC	TNTC	9	1	0
	TNTC	TNTC	5	0	0
	TNTC	TNTC	7	4	0
	TNTC	TNTC	13	2	0
	TNTC	TNTC	11	3	0
	TNTC	TNTC	9	1	0
	TNTC	TNTC	7	3	0
	TNTC	TNTC	10	0	0
200 mg	1	0	0	0	0
	2	0	0	0	0
	1	0	0	0	0
	1	0	0	0	0
	0	0	0	0	0
	3	0	0	0	0
	2	0	0	0	0
	0	0	0	0	0
	1	0	0	0	0
	1	0	0	0	0
	1	0	0	0	0
	0	0	0	0	0
	2	0	0	0	0
	0	0	0	0	0
	4	0	0	0	0

CFU/mL:
0 -1 -2 -3 -4 None N/A N/A 9.0E+04 3.0E+05 N/A N/A N/A 9.0E+04 1.0E+05 N/A N/A N/A 1.1E+05 9.0E+05 N/A N/A N/A 1.3E+05 3.0E+05 N/A N/A N/A 9.0E+04 2.0E+05 N/A N/A N/A 1.3E+05 2.0E+05 N/A N/A N/A 1.6E+05 3.0E+05 N/A N/A N/A 9.0E+04 1.0E+05 N/A N/A N/A 5.0E+04 0.0E+00 N/A N/A N/A 7.0E+04 4.0E+05 N/A N/A N/A 1.3E+05 2.0E+05 N/A N/A N/A 1.1E+05 3.0E+05 N/A N/A N/A 9.0E+04 1.0E+05 N/A N/A N/A 7.0E+04 3.0E+05 N/A N/A N/A 1.0E+05 0.0E+00 N/A 200 mg 1.0E+01 N/A N/A N/A N/A 2.0E+01 N/A N/A N/A N/A 1.0E+01 N/A N/A N/A N/A 1.0E+01 N/A N/A N/A N/A 0.0E+00 N/A N/A N/A N/A 3.0E+01 N/A N/A N/A N/A 2.0E+01 N/A N/A N/A N/A 0.0E+00 N/A N/A N/A N/A 1.0E+01 N/A N/A N/A N/A 1.0E+01 N/A N/A N/A N/A 1.0E+01 N/A N/A N/A N/A 0.0E+00 N/A N/A N/A N/A 2.0E+01 N/A N/A N/A N/A 0.0E+00 N/A N/A N/A N/A 4.0E+01 N/A N/A N/A N/A

Averages:

	0	-1	-2	-3	-4
None
Avg	N/A	N/A	1.0E+05	2.5E+05	N/A
Std	N/A	N/A	2.8E+04	2.2E+05	N/A
200 mg
Avg	1.3E+01	N/A	N/A	N/A	N/A
Std	1.2E+01	N/A	N/A	N/A	N/A

The average recovery of spores from the No ClO2 exposure group is listed above, meaning that the recovery of the P. larvae spores from the glass cover slip was between 35.7% to 89.3% (from the 2.8x10^5 CFU/mL). Which isn't too bad, but not the best either.

Graph (using the -2 data from the No ClO2 group since it had the lowest Std)

//EWW