ClO2 Study - P. larvae Spores on Glass

From 4-23-14.

I was able to count the P. larvae colonies that grew on the MYPGP agar plates after being exposed to 12.5 mg of ClO2 reagent.

The CFU/mL was calculated using the formula:

One of the MYPGP dilution plates with the P. larvae on it that had been exposed to 12.5 mg of ClO2

How to calculate CFU/mL

(CFU * Dilution Factor * Volume Factor)

or

(# CFU) * ( 1 / 10^ dilution factor) * ( 1 / volume plated in mL) = CFU / mL

ex:

3 * ( 1 / 10^ (-4)) *  ( 1 / 0.01mL) = 3x10^6 CFU /mL

The data is stored in an Excel file.