Wax moth eggs were transferred to new containers with diet. Eggs were present on the tops of the containers near where the lid of the container attaches. A number of the eggs were present on the wax paper at that interface. A hodgepodge of containers were used for new eggs. Most of the wax worms have almost completed pupation and are laying eggs, so I'll continue to monitor and transfer eggs as I see them.
Contamination:
There were two large secondary containers each with four smaller containers within. The containers were stored in the 30C walk in incubator, but the container that was stored in the secondary corner of the room had three of its four smaller containers contaminated with fungus! The other secondary container that was in the middle of the shelf had no fungal growth. Not a big problem with the survival of the colony as there are still PLENTY of eggs in the other containers. In fact, some of the eggs were stored in an Eppendorf tube at 4C.
It would appear that conditions were different enough in the 30C incubator depending on where the containers were that it caused fungal growth. In the future, I will only store the containers in the middle of the shelf just to be safe.
//EWW
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