I am able to count the CFU on the MYPGP from the spores on the metal coupons that were exposed to ClO2 gas for three hours. Unfortunately, I wasn't able to count any colonies from the treatment groups (even from the 10^-0 dilution!) as no colonies grew on the plates.
Spore stock #11 was added to the coupons, which means there was about 1.8x10^5 CFU on the coupon when it was exposed to the ClO2 gas. I was able to count CFU from the spores on metal that were exposed to ClO2 gas, unfortunately it was 2.2x10^4 CFU/mL, which means there was only about a 12% rate of recovery of the spore from the coupons! This was a similar problem that I had with the spores on wood, the spore recovery is very poor. I will need to adjust my re-suspension protocol for these two surfaces (pine chips and metal coupons). Perhaps increasing the time the surfaces are soaked in the 1 mL H2O would help with re-suspension.
Below is an image of one of the MYPGP dilution plates. There were only two colonies on the plate from the 10^-0 dilution. However, duplicate plates did not have any colony growth on them.
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| Spores on metal exposed to 50 mg weight of ClO2 reagent for 3 hours |
//EWW
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