From 8-8-14.
On August 9th (day 2) - transferred each larva to a new well in the 24 well plate and fed each larva 10 uL BAD. There doesn't appear to be any deaths at this time point, although it is more difficult to determine at this early instar due to a general lack of movement and glistening caused by the BAD.
On August 10th (day 3) - Fed each larva an additional 20 uL of BAD. Identified one dead larva out of the 12.
On August 11th (day 4) - Fed each larva an additional 30 uL of BAD. No new deaths were observed in any of the larva.
On August 12th (day 5) - Each larva was fed an additional 40 uL of BAD. An additional death was observed. This takes the total dead to two larva out of 12 (16.7%).
These results so far are interesting. According to my finding in the P. larvae LC50 assay on 8-3-14 the first instar honey bee larva that were fed 1000 CFU/mL of P. larvae spores displayed about 50% death by day 5. Here in this experiment there is only 16.7% death after 5 days. In this experiment the first instar larva were fed 1000 CFU/mL of P. larvae spores (from the same stock as the LC50 assay) along with 1000 cells/mL of yeast. Could it be that the yeast had some sort of inhibitory effect on the spores or their virulence? I will continue to monitor survival.
This experiment with the spores/yeast was my attempt at salvaging my initial attempts after discovering a significantly less amount of available honey bee larva. This is an excellent preliminary glimpse, however this experiment should obviously be repeated with a larger sample size and controls!
//EWW
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