Discovered that I had miscounted the number of honey bee larva for this experiment! I originally thought I had 12 total, but in reality I only had 10. Not a big problem, and it certainly didn't effect how the larva were handled during this experiment. The survival percentages just need to be recalculated.
The last larva has died here on day 10 (10/10 larva dead). This marks the last day of this preliminary experiment. With the new and correct number larva in this study here is the breakdown of results:
| n=10 | Died | % Survival |
| Day 1 | 0 | 100.0% |
| Day 2 | 0 | 100.0% |
| Day 3 | 1 | 90.0% |
| Day 4 | 0 | 90.0% |
| Day 5 | 1 | 80.0% |
| Day 6 | 1 | 70.0% |
| Day 7 | 0 | 70.0% |
| Day 8 | 4 | 30.0% |
| Day 9 | 2 | 10.0% |
| Day 10 | 1 | 0.0% |
Quick graph of data:
Compare to P. larvae LC50 Study. There was a reduced mortality rate compared to the P. larvae LC50 study during the early instars, however the mortality by day 9 (end of LC50 study) was similar. Overall, it doesn't appear that there was any effect on the survival of honey bee larva when fed 1000 CFU/mL P. larvae spores with or without the addition of 1000 Cells/mL of yeast. It could be that the yeast had a delaying effect on the P. larvae as the % mortality seen in this study was reduced during the first 6 days compared to the LC50 study. This study would need to be repeated on a larger scale to make a more accurate evaluation of the effect of yeast on P. larvae's ability to cause disease in honey bee larva.
//EWW
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