From 8-3-14.
Methods paper for rearing waxworms/moths in vitro from COLOSS.
Small clusters of waxworm eggs were discovered on the folded waxpaper from a container created on 8-3-14. Eggs were carefully teased apart and transferred to a 48 well plate using a camel hair paint brush and sharp forceps.
12 eggs were transferred to 2 uL of BAD
6 eggs were transferred to 5 uL of BAD
6 eggs were transferred to 10 uL of BAD
48 well plate was incubated at 35C in anaerobic chamber with ~90% humidity. I will monitor their progress daily.
//EWW
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