Surprisingly there was one dead cockroach from the 10,000 CFU/mL P. larvae spore treatment group. I would sterilize the outside of the roach (bleach) and grind it up for PCR analysis using the method described here, but unfortunately there was also a dead roach in the control group!
I will continue to monitor their survival, if the remaining two roaches in the 10,000 CFU/mL group die in the near future and the control group remains alive then perhaps I will further investigate the cause of death. For now, the dead roaches were frozen at -20C and will be disposed of tomorrow morning.
Treatment-alive after 48 hours
200 CFU/mL - 3
10,000 CFU/mL - 2
Control (no spores) - 2
//EWW
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