P. larvae growth in BHI and H2O was beyond what could be counted on these plates (they were further diluted on 7-5-14 and those plates will be examined at a future date). P. larvae growth on the BAD was reduced. This could be due to the antimicrobial capabilities of the Royal Jelly, that comprised 53% of the BAD. This may indicate that the P. larvae spores have a reduced germination capability on the BAD. This was very interesting and I will confirm these findings in future studies.
Dr. Fisher provided me with a brief description of spore structure and gave me some directional ideas regarding this particular project. Pictures of the white board discussion are posted below.
A good video that discusses nearly everything that was discussed with Dr. Fisher is located here. Remember, sporulation is when a vegetative cell create a spore and germination is when that spore develops into a vegetative cell.
Future:
Repeat entire experiment to confirm results as well as to check if P. larvae spores are able to germinate in the BAD. This will be accomplished by heating each treatment group at 65C for 15 minutes after the incubation period. This heating will kill off any spores that did germinate and became vegetative cells. Meaning, if there is a lower CFU count after the heating process as opposed to no-heat, then we can conclude that the spores did germinate.
//EWW
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