P. larvae Project Pilot

Added 30 uL of BAD to all honey bee larva in the 24 well plates. It appears that the majority of larva had consumed most if not all the present BAD. Added 10 uL to the eggs/larva that was in the 96 well plates (they do not look like they are doing well). Two pictures of larva growing in the 24 well plates is shown below:

Moved the spot plates from 6-30-14 from the static 37C incubator to the 37C 5%CO2 incubator and inverted them. The P. larvae grows better with the CO2 concentration.

Began to isolate spores from 1:100 dilution of P. larvae on MYPGP plate from 6-21-14 (10 days of incubating at 37C 5%CO2), but stopped after the first centrifugation. Barely any pellet was seen (if any) and little culture was recovered from the agar plate. This may be due to the fact the plate had been incubated for 10 days. It is recommended to only incubate the plates 6-7 and then recovered spores as that is peak spore production. Discarded the sample. I will only attempt to harvest spores after the 6-7 days of incubation in the future.

//EWW