P. larvae Project Pilot

Added 50 uL of BAD to all honey bee larva in the 24 well plates. This is the last time I will feed them before they begin to pupate. After the bee larva has finished their 6th instar feeding life stage they will have a large defecation stage and then begin to pupate, creating a very thin cocoon. The bee larva will be left in the 24 well plates and incubated at 35C during the entirety of their pupation stage. These larva have been incubating since June. Image of what they look like in the 24 well plates is shown below:

The larva will begin to straighten out once all the food is consumed, defecate, then begin to pupate into honey bees.

Attempted to look at Spore Stocks under confocal light microscopy, but didn't see many spores. There was a substantial amount of cellular debris seen in Spore Stock A and relatively few spores.
I need to find a microscope with phase contrast capabilities, but I don't think any microscope in Van Es has that feature.

Inoculated 100 uL of the 10^-1 dilution of Spore Stocks B and C onto MYPGP agar. Plates were incubated at 35C 5%CO2 not inverted. I will attempt to isolate spore stocks from these samples 6-7 days from now.

//EWW