P. larvae PCR Identification

Repeated P. larvae PCR Identification procedure using the lysates frozen at -20C.

Key: 

Top Lanes- AFB primers
1	2	3	4	5	6	7	8	9	10

Code	Sample
2	Spore Stock A
3	Spore Stock B
4	Spore Stock C
5	Plate B (7-5-14)
6	Plate C (7-5-14)
7	Drop Plate - BHI Treatment (7-3-14)
8	Drop Plate - H2O Treatment (7-3-14)
9	Drop Plate - B.A.D. Treatment (7-3-14)

Lanes 1 and 10 contained the Hi-Lo DNA Markers

My suspicions of the first time I amplified these samples was true, all spore stocks did indeed amplify using these P. larvae specific primers (Stocks A, B, and C). More interestingly, Sample 9 did not amplify, indicating that it is not P. larvae. This sample was from a colony on the drop plate of P. larvae incubated with Bee Artificial Diet (BAD) in the experiment to see if P. larvae can germinate in that medium. There was definitely bacteria that grew on the MYPGP agar, but apparently it wasn't P. larvae... Maybe it was another bacteria, or maybe there was human error when selecting a colony. Regardless, it was very interesting that that particular sample didn't test positive for P. larvae.

//EWW